To obtain a precise measurement of gene expression, it is essential to normalize the results of your real time PCR in relation to the fixed expression of the reference gene in such away that experimental conditions do not affect the result.
Although standardization with reference to the constituent expression of the housekeeping gene is the most common method, there is no universal reference gene that is constant in all experimental situations.
For example, the levels of expression of a reference gene can vary for the following reasons:
With a correct standardization strategy, it can be more precise, reproducible and sensitive in your real time PCR.
geNorm is a system for selecting the best candidate reference gene for certain experimental conditions. Simply quantify the 6 or 12 housekeeping genes in your own samples, and together with the geNorm software included in your Kit you will be able to calculate which housekeeping gene is expressed with the most stability in your experimental model.
The geNorm Kits are available for a large number of species. If you need a geNorm Kit for a species that is not in the list, you can simply request one.
More than 1000 scientific publications have cited the geNorm method.
Kit contents:
The Kit contains enough reactive for 10 geNorm analyses.
Price: Make an order
When ordering, please indicate the specie and whether you prefer SYBR green or PerfectProbe. If you are purchasing a 6 gene kit then you can select the 6 genes you would like in the kit.